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14 April 2006 Histological observation on dental hard tissue irradiated by ultrashort-pulsed laser
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In the field of dentistry, effectiveness of USPL irradiation is researched because USPL has less thermal side effect to dental hard tissue. In this paper, we observed morphological change and optical change of dental hard tissue irradiated by USPL for discussing the safety and effectiveness of USPL irradiation to dental hard tissues. Irradiated samples were crown enamel and root dentin of bovine teeth. Lasers were Ti:sapphire laser, which had pulse duration (Pd)of 130 fsec and pulse repetition rate (f) of 1kHz and wavelength (l) of 800nm, free electron laser (FEL), which had Pd of 15 μsec and f of 10Hz and wavelength of 9.6μm, and Er:YAG laser, which had Pd of 250 μsec and f of 10Hz and wavelength of 2.94μm. After laser irradiation, the sample surfaces and cross sections were examined with SEM and EDX. The optical change of samples was observed using FTIR. In SEM, the samples irradiated by USPL had sharp and accurate ablation with no crack and no carbonization. But, in FEL and Er:YAG laser, the samples has rough ablation with crack and carbonization. It was cleared that the P/Ca ratio of samples irradiated by USPL had same value as non-irradiated samples. There was no change in the IR absorption spectrum between samples irradiated by USPL and non-irradiated sample. But, they of samples irradiated by FEL and Er:YAG laser, however, had difference value as non-irradiated samples. These results showed that USPL might be effective to ablate dental hard tissue without thermal damage.
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Takeyuki Uchizono, Kunio Awazu, Akihiro Igarashi, Junji Kato D.D.S., and Yoshito Hirai D.D.S. "Histological observation on dental hard tissue irradiated by ultrashort-pulsed laser", Proc. SPIE 6191, Biophotonics and New Therapy Frontiers, 619102 (14 April 2006); doi: 10.1117/12.661925;

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