Application of fluorescence fluctuation spectroscopy to the measurement of the concentration of molecules deposited on solid substrates

Jacques Derouard; Antoine Delon; Rodolphe Jaffiol; Cyrille Vézy

doi:10.1117/12.662294

14 April 2006 Application of fluorescence fluctuation spectroscopy to the measurement of the concentration of molecules deposited on solid substrates

Jacques Derouard, Antoine Delon, Rodolphe Jaffiol, Cyrille Vézy

Author Affiliations +

Proceedings Volume 6191, Biophotonics and New Therapy Frontiers; 61910C (2006) https://doi.org/10.1117/12.662294
Event: SPIE Photonics Europe, 2006, Strasbourg, France

Abstract

Fluorescence fluctuation spectroscopy is applied to study molecules, passing through a small observation volume, usually subjected to diffusive or convective motion in liquid phase. We suggest that such a technique could be used to measure the areal absolute concentration of fluorophores deposited on a substrate or imbedded in a thin film, with a resolution of a few micrometers. The principle is to translate the solid substrate in front of a confocal fluorescence microscope objective and to record the subsequent fluctuations of the fluorescence intensity. The validity of this concept is investigated on model substrates (fluorescent microspheres), DNA-chips, and dye-stained histidine molecules anchored on silanized glass surfaces.

Citation Download Citation

Jacques Derouard, Antoine Delon, Rodolphe Jaffiol, and Cyrille Vézy "Application of fluorescence fluctuation spectroscopy to the measurement of the concentration of molecules deposited on solid substrates", Proc. SPIE 6191, Biophotonics and New Therapy Frontiers, 61910C (14 April 2006); https://doi.org/10.1117/12.662294

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available