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14 April 2006 Application of fluorescence fluctuation spectroscopy to the measurement of the concentration of molecules deposited on solid substrates
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Abstract
Fluorescence fluctuation spectroscopy is applied to study molecules, passing through a small observation volume, usually subjected to diffusive or convective motion in liquid phase. We suggest that such a technique could be used to measure the areal absolute concentration of fluorophores deposited on a substrate or imbedded in a thin film, with a resolution of a few micrometers. The principle is to translate the solid substrate in front of a confocal fluorescence microscope objective and to record the subsequent fluctuations of the fluorescence intensity. The validity of this concept is investigated on model substrates (fluorescent microspheres), DNA-chips, and dye-stained histidine molecules anchored on silanized glass surfaces.
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Jacques Derouard, Antoine Delon, Rodolphe Jaffiol, and Cyrille Vézy "Application of fluorescence fluctuation spectroscopy to the measurement of the concentration of molecules deposited on solid substrates", Proc. SPIE 6191, Biophotonics and New Therapy Frontiers, 61910C (14 April 2006); https://doi.org/10.1117/12.662294
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