14 April 2006 Detection of colorectal cancer using time-resolved autofluorescence spectrometer
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Abstract
As we know Quantum mechanics is a mathematical theory that can describe the behavior of objects that are at microscopic level. Time-resolved autofluorescence spectrometer monitors events that occur during the lifetime of the excited state. This time ranges from a few picoseconds to hundreds of nanoseconds. That is an extremely important advance as it allows environmental parameters to be monitored in a spatially defined manner in the specimen under study. This technique is based on the application of Quantum Mechanics. This principle is applied in our project as we are trying to use different fluorescence spectra to detect biological molecules commonly found in cancerous colorectal tissue and thereby differentiate the cancerous and non-cancerous colorectal polyps more accurately and specifically. In this paper, we use Fluorescence Lifetime Spectrometer (Edinburgh Instruments FL920) to measure decay time of autofluorescence of colorectal cancerous and normal tissue sample. All specimens are from Department of Colorectal Surgery, Singapore General Hospital. The tissues are placed in the time-resolved autofluorescence instrument, which records and calculates the decay time of the autofluorescence in the tissue sample at the excitation and emission wavelengths pre-determined from a conventional spectrometer. By studying the decay time,τ, etc. for cancerous and normal tissue, we aim to present time-resolved autofluorescence as a feasible technique for earlier detection of malignant colorectal tissues. By using this concept, we try to contribute an algorithm even an application tool for real time early diagnosis of colorectal cancer for clinical services.
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Sheng Fu, Leong-Chuan Kwek, Teck-Chee Chia, Chu-Sing Lim, Choong-Leong Tang, Wuan-Suan Ang, Miao-Chang Zhou, Po-Ling Loke, "Detection of colorectal cancer using time-resolved autofluorescence spectrometer", Proc. SPIE 6191, Biophotonics and New Therapy Frontiers, 61910M (14 April 2006); doi: 10.1117/12.663257; https://doi.org/10.1117/12.663257
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