14 April 2006 Digital holographic microscopy: a new optical imaging technique to investigate cellular dynamics
Author Affiliations +
Abstract
We have developed a digital holographic microscope (DHM), in a transmission mode, adapted to the quantitative study of cellular dynamics. Living cells are optically probed by measuring the phase shift they produce on the transmitted wave front. The high temporal stability of the phase signal, equivalent to λ/1800, and the low acquisition time (down to 20 μs) enables to monitor cellular dynamics processes. An experimental procedure allowing to calculate both the intracellular refractive index and the cellular thickness (morphometry) from the measured phase shift is presented.
© (2006) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Pierre Marquet, Pierre Marquet, Benjamin Rappaz, Benjamin Rappaz, Tristan Colomb, Tristan Colomb, Florian Charrière, Florian Charrière, Jonas Kühn, Jonas Kühn, Yves Emery, Yves Emery, Etienne Cuche, Etienne Cuche, Christian Depeursinge, Christian Depeursinge, Pierre Magistretti, Pierre Magistretti, } "Digital holographic microscopy: a new optical imaging technique to investigate cellular dynamics", Proc. SPIE 6191, Biophotonics and New Therapy Frontiers, 61910U (14 April 2006); doi: 10.1117/12.662988; https://doi.org/10.1117/12.662988
PROCEEDINGS
5 PAGES


SHARE
Back to Top