Glass based fluorescence reference materials used for optical and biophotonic applications

A. Engel; C. Ottermann; U. Resch-Genger; K. Hoffmann; S. Schweizer; J. Selling; J.-M. Spaeth; V. Rupertus

doi:10.1117/12.663627

14 April 2006 Glass based fluorescence reference materials used for optical and biophotonic applications

A. Engel, C. Ottermann, U. Resch-Genger, K. Hoffmann, S. Schweizer, J. Selling, J.-M. Spaeth, V. Rupertus

Proceedings Volume 6191, Biophotonics and New Therapy Frontiers; 619110 (2006) https://doi.org/10.1117/12.663627
Event: SPIE Photonics Europe, 2006, Strasbourg, France

Abstract

Fluorescence techniques are known for their high sensitivity and are widely used as analytical tools and detection methods for product and process control, material sciences, environmental and biotechnical analysis, molecular genetics, cell biology, medical diagnostics, and drug screening. For routine measurements by fluorescence techniques the existence of an improved quality assurance is one of the basic needs. According to DIN/ISO 17025 certified standards are used for fluorescence diagnostics having the drawback of giving relative values only. Typical requirements onto fluorescence reference materials or standards deal with the verification of the instrument performance as well as the improvement of the data comparability. Especially for biomedical applications fluorescence labels are used for the detection of proteins. In particular these labels consist of nano crystalline materials like CdS and CdSe. The field of Non-Cadmium containing materials is under investigation. In order to evaluate whether glass based materials can be used as standards it is necessary to calculate absolute values like absorption/excitation cross sections or relative quantum yields. This can be done using different quantities of dopands in glass, glass ceramics or crystals. The investigated materials are based on different types of glass, silicate, phosphate and boron glass, which play a dominant role for the absorption and emission mechanism. Additional to the so-called elementary fluorescence properties induced by raw earth elements the formation of defects lead to higher cross sections additionally. The main investigations deal with wavelength accuracy and lifetime of doped glasses, glass ceramics and crystalline samples. Moreover intensity patterns, homogeneity aspects and photo stability will be discussed.

Citation Download Citation

A. Engel, C. Ottermann, U. Resch-Genger, K. Hoffmann, S. Schweizer, J. Selling, J.-M. Spaeth, and V. Rupertus "Glass based fluorescence reference materials used for optical and biophotonic applications", Proc. SPIE 6191, Biophotonics and New Therapy Frontiers, 619110 (14 April 2006); https://doi.org/10.1117/12.663627

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available