12 September 2006 Laser-induced autofluorescence properties of base-cell lesions: analysis and algorithms for diagnosis and differentiation
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The goals of this work were investigation of base-cell skin lesions by the method of laser-induced autofluorescence spectroscopy. Fluorescence spectra were obtained from benign base-cell papilloma and malignant base-cell carcinoma, as well as from healthy skin areas near to the lesions that were used posteriori to reveal changes between healthy and lesion skin spectra. Preliminarily lesions were classified by dermatoscopic method (MoleMax II, DERMA Instruments). All suspicious lesions were excised and were investigated histologically. The experimental set-up consists of a nitrogen laser (337 nm, 14 μJ, 10 Hz), lenses, filters, optical fibers, and a microspectrometer (PC2000, "Ocean Optics"). A computer controls this system. Spectrum of healthy skin consists of one main maximum at 470-500 nm spectral region and secondary maxima at in the regions around 400 and 440 nm. In cases of papilloma and base-cell carcinoma an intensity decrease was observed, related to accumulation of pigments in these cutaneous lesions. An relative increase of the fluorescence peak at 440 nm were registered in the case of base-cell carcinoma, related to metabolism activity increase, and appearance of green fluorescence, related to increase of keratin content in benign papilloma lesions were detected. The results, obtained were used to develop multispectral diagnostic algorithm of these base-cell lesions. An sensitivity of 89,4% and 91,0% and specificity of 99,6% and 97,4% for differentiation between normal skin and papilloma and carcinoma respectively were obtained. The capability of the human skin fluorescence spectroscopy for early diagnosis and differentiation of cutaneous lesions is shown.
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E. Borisova, E. Borisova, P. Troyanova, P. Troyanova, L. Avramov, L. Avramov, } "Laser-induced autofluorescence properties of base-cell lesions: analysis and algorithms for diagnosis and differentiation", Proc. SPIE 6284, International Conference on Lasers, Applications, and Technologies 2005: Laser Technologies for Environmental Monitoring and Ecological Applications, and Laser Technologies for Medicine, 62840F (12 September 2006); doi: 10.1117/12.714159; https://doi.org/10.1117/12.714159

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