The goals of this work were investigation of base-cell skin lesions by the method of laser-induced autofluorescence
spectroscopy. Fluorescence spectra were obtained from benign base-cell papilloma and malignant base-cell carcinoma,
as well as from healthy skin areas near to the lesions that were used posteriori to reveal changes between healthy and
lesion skin spectra. Preliminarily lesions were classified by dermatoscopic method (MoleMax II, DERMA Instruments).
All suspicious lesions were excised and were investigated histologically. The experimental set-up consists of a nitrogen
laser (337 nm, 14 μJ, 10 Hz), lenses, filters, optical fibers, and a microspectrometer (PC2000, "Ocean Optics"). A
computer controls this system.
Spectrum of healthy skin consists of one main maximum at 470-500 nm spectral region and secondary maxima at in the
regions around 400 and 440 nm. In cases of papilloma and base-cell carcinoma an intensity decrease was observed,
related to accumulation of pigments in these cutaneous lesions. An relative increase of the fluorescence peak at 440 nm
were registered in the case of base-cell carcinoma, related to metabolism activity increase, and appearance of green
fluorescence, related to increase of keratin content in benign papilloma lesions were detected. The results, obtained were
used to develop multispectral diagnostic algorithm of these base-cell lesions. An sensitivity of 89,4% and 91,0% and
specificity of 99,6% and 97,4% for differentiation between normal skin and papilloma and carcinoma respectively were
obtained. The capability of the human skin fluorescence spectroscopy for early diagnosis and differentiation of cutaneous
lesions is shown.