Novel europium-tetracycline probe for phosphate determination in microtiter plate

Matejka Turel; Aleksandra Lobnik; Axel Duerkop; Otto S. Wolfbeis

doi:10.1117/12.714179

12 September 2006 Novel europium-tetracycline probe for phosphate determination in microtiter plate

Matejka Turel, Aleksandra Lobnik, Axel Duerkop, Otto S. Wolfbeis

Proceedings Volume 6284, International Conference on Lasers, Applications, and Technologies 2005: Laser Technologies for Environmental Monitoring and Ecological Applications, and Laser Technologies for Medicine; 62840M (2006) https://doi.org/10.1117/12.714179
Event: International Conference on Lasers, Applications, and Technologies 2005, 2005, St. Petersburg, Russian Federation

Abstract

A new luminescent europium probe is presented for the determination of phosphate (P) in microtiter plate format. The assay is based on the quenching of the luminescence of the europium-tetracycline 1:1 complex by phosphate using a reagent concentration of 20.8 μmol/L. The probe is excited at 400 nm and displays large Stokes' shift of 210 nm. The emission maximum is located at 616 nm. The system works best at neutral pH 7. The linear range of the calibration plot is from 5•10^-6 mol/L to 7.5•10^-4 mol/L of phosphate, and the limit of detection is 5μmol/L.

Citation Download Citation

Matejka Turel, Aleksandra Lobnik, Axel Duerkop, and Otto S. Wolfbeis "Novel europium-tetracycline probe for phosphate determination in microtiter plate", Proc. SPIE 6284, International Conference on Lasers, Applications, and Technologies 2005: Laser Technologies for Environmental Monitoring and Ecological Applications, and Laser Technologies for Medicine, 62840M (12 September 2006); https://doi.org/10.1117/12.714179

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