5 September 2006 CCD on-chip imaging for smart micro-object and fluorescence imaging system
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The imaging method by simply putting the object directly on the surface of CCD or CMOS array chip is experimented. A 4.65*4.65μm pixel size 1024*768 CCD chip is used in the experiments. CCD's optic window is removed and surrounding lead on chip is covered with some glue to protect the CCD chip from damaged during the imaging operation. Light emitted or transmitted from object is gathered by the nearest one or several pixels. The comparative experiment shows, to compare the normal microscope imaging using the optic lens, Sharp image with no color shift, optic aberration and field distortion is achieved. To compare with a 4x N.A=0.1 lens at the same magnification, better light couple efficiency and simple optomechanics construction are achieved in our experiment system. As the key factors effecting the image PSF,MTF, resolution, contrast and couple efficiency, the object distance and illumination light path are analyzed and experimented. It shows this will be a potential ultra small size, high efficiency and low cost micro-object imaging and fluorescence imaging system for cell level biology photometry and imaging.
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Hongyun Qiu, Hongyun Qiu, Yang Liu, Yang Liu, Liqun Sun, Liqun Sun, Enyao Zhang, Enyao Zhang, Qian Tian, Qian Tian, } "CCD on-chip imaging for smart micro-object and fluorescence imaging system", Proc. SPIE 6289, Novel Optical Systems Design and Optimization IX, 628913 (5 September 2006); doi: 10.1117/12.679977; https://doi.org/10.1117/12.679977


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