For multispectral imagery (MSI), spatial registration between bands is a very important part of the overall quality of the MSI product. For some remote sensing imagery, mis-registration of bands greater than about one-quarter of a pixel can be visually noticeable. Based on the successful registration processing developed for the Multispectral Thermal Imager (MTI) (known as edgereg), a derivative algorithm was developed for assessment of spatial registration accuracy of 4-band MSI. This algorithm has sub-pixel accuracy of a few hundredths of a pixel, and can be used to compute an image average mis-registration or process imagery in a block mode to create a mis-registration map. This paper presents the results of testing the algorithm on simulated and commercial imagery.