Chemically sensitive 3D imaging at sub-100-nm spatial resolution using tomography in a scanning transmission x-ray microscope

Göran A. Johansson; James J. Dynes; Adam P. Hitchcock; Tolek Tyliszczak; George D. W. Swerhone; John R. Lawrence

doi:10.1117/12.681217

7 September 2006 Chemically sensitive 3D imaging at sub-100-nm spatial resolution using tomography in a scanning transmission x-ray microscope

Göran A. Johansson, James J. Dynes, Adam P. Hitchcock, Tolek Tyliszczak, George D. W. Swerhone, John R. Lawrence

Author Affiliations +

Proceedings Volume 6318, Developments in X-Ray Tomography V; 63181I (2006) https://doi.org/10.1117/12.681217
Event: SPIE Optics + Photonics, 2006, San Diego, California, United States

Abstract

Computed angle scan tomography has been implemented for the first time using sample raster scanning in a scanning transmission X-ray microscope. The experimental apparatus, acquisition and data analysis procedures, and first results from fully hydrated river biofilm samples measured from 528-534 eV are reported. The multiple energy images are processed to 3-dimensional quantitative chemical maps of major biological components, such as proteins, polysaccharides and lipids.

Citation Download Citation

Göran A. Johansson, James J. Dynes, Adam P. Hitchcock, Tolek Tyliszczak, George D. W. Swerhone, and John R. Lawrence "Chemically sensitive 3D imaging at sub-100-nm spatial resolution using tomography in a scanning transmission x-ray microscope", Proc. SPIE 6318, Developments in X-Ray Tomography V, 63181I (7 September 2006); https://doi.org/10.1117/12.681217

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available