7 September 2006 Chemically sensitive 3D imaging at sub-100-nm spatial resolution using tomography in a scanning transmission x-ray microscope
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Abstract
Computed angle scan tomography has been implemented for the first time using sample raster scanning in a scanning transmission X-ray microscope. The experimental apparatus, acquisition and data analysis procedures, and first results from fully hydrated river biofilm samples measured from 528-534 eV are reported. The multiple energy images are processed to 3-dimensional quantitative chemical maps of major biological components, such as proteins, polysaccharides and lipids.
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Göran A. Johansson, Göran A. Johansson, James J. Dynes, James J. Dynes, Adam P. Hitchcock, Adam P. Hitchcock, Tolek Tyliszczak, Tolek Tyliszczak, George D. W. Swerhone, George D. W. Swerhone, John R. Lawrence, John R. Lawrence, } "Chemically sensitive 3D imaging at sub-100-nm spatial resolution using tomography in a scanning transmission x-ray microscope", Proc. SPIE 6318, Developments in X-Ray Tomography V, 63181I (7 September 2006); doi: 10.1117/12.681217; https://doi.org/10.1117/12.681217
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