11 September 2006 Manipulation of yeast cells in a microfluidic channel using the GPC-based optical trapping system
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Abstract
In the area of manipulating microscopic biological specimen, optical trapping has proven its worth. Still, many potential microbiological applications can benefit when the experiment is assisted by a computer and capable of running either with only limited supervision or full automation. Here we have implemented the Generalized Phase Contrast (GPC) method of optical trapping in a microfluidic system, and show how an experiment can be easily made to run autonomously, while the computer continuously adapts the light pattern to trap yeast cells passing through the trapping volume. The optical trapping takes place in a microfluidic system where two channels meet, allowing for separate injection of specimen and work media. Yeast cells are trapped near the surface of the microchannel at flow rates that give particle speeds of more than 50 μm/s. We demonstrate the ability of GPC-based traps to hold the cells in specific positions and observe the displacement of the cells from respective trap centers. Finally we exploit the speed of the GPC system by dynamically detect yeast cells using a CCD camera and immediately create traps at their positions at flow rates that exceed what a human operator would be able to handle. The optical system was found to be easily expanded and the attention could be kept on maintaining optimal conditions for the yeast.
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Ivan R. Perch-Nielsen, Ivan R. Perch-Nielsen, Peter John Rodrigo, Peter John Rodrigo, Jesper Glückstad, Jesper Glückstad, "Manipulation of yeast cells in a microfluidic channel using the GPC-based optical trapping system", Proc. SPIE 6326, Optical Trapping and Optical Micromanipulation III, 63261X (11 September 2006); doi: 10.1117/12.681515; https://doi.org/10.1117/12.681515
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