3 November 2006 Time-correlated single photon counting: an advancing technique in a plate reader for assay development and high throughput screening
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Proceedings Volume 6372, Advanced Photon Counting Techniques; 637208 (2006) https://doi.org/10.1117/12.688419
Event: Optics East 2006, 2006, Boston, Massachusetts, United States
Abstract
A new plate reader (Nanotaurus) has been developed by Edinburgh Instruments that has the principle design features of a confocal microscope and utilises the technique of Time Correlated Single Photon Counting for data acquisition. The advantages of Fluorescence Lifetime Measurements in the nanosecond time scale and analysis methods to recover lifetime parameters are discussed based on experimental data. First working assays using changes of lifetime parameters are presented that clearly demonstrate the advantages of the new instrument for biochemical assays and show strong promise for cell-based assays, by utilising the independence of lifetime parameters from sample volume and concentration.
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Dirk U. Näther, Dirk U. Näther, Roger Fenske, Roger Fenske, Reynald Hurteaux, Reynald Hurteaux, Sandra Majno, Sandra Majno, S. Desmond Smith, S. Desmond Smith, } "Time-correlated single photon counting: an advancing technique in a plate reader for assay development and high throughput screening", Proc. SPIE 6372, Advanced Photon Counting Techniques, 637208 (3 November 2006); doi: 10.1117/12.688419; https://doi.org/10.1117/12.688419
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