Imaging technology of three dimensional distribution for sugar chain on single living cell membrane

Kazuya Yamamoto; Ichirou Ishimaru; Yoshiki Fujii; Toshiki Yasokawa; Katsumi Ishizaki; Makoto Yoshida; Kaoru Takegawa; Naotaka Tanaka; Shigeki Kuriyama; Tsutomu Masaki; Seiji Nakai

doi:10.1117/12.685893

19 October 2006 Imaging technology of three-dimensional distribution for sugar chain on single living cell membrane

Kazuya Yamamoto, Ichirou Ishimaru, Yoshiki Fujii, Toshiki Yasokawa, Katsumi Ishizaki, Makoto Yoshida, Kaoru Takegawa, Naotaka Tanaka, Shigeki Kuriyama, Tsutomu Masaki, Seiji Nakai

Author Affiliations +

Proceedings Volume 6374, Optomechatronic Actuators, Manipulation, and Systems Control; 63740L (2006) https://doi.org/10.1117/12.685893
Event: Optics East 2006, 2006, Boston, Massachusetts, United States

Abstract

We study on the imaging technology of three-dimensional distribution for sugar chain on single living cell-membrane. This technology can observe the entire cell surface. To observe the cell surface, the local area image of cell-membrane is taken by TIRF (total internal reflection fluorescence) microscopy. And by scanning the whole cell surface area, we can obtain the image of the entire cell membrane. These observed local area images can be converted into an entire surface image by the pattern matching processing. For this scanning technology, we propose the proximal two beam optical tweezers to rotate the single floating cell. This proximal two beam optical tweezers can rotate the floating single cell in the nutrient medium by light pressure. Two beams illuminate the single cell at proximal two points from below and above. The cell is trapped at the center of these two focal points. At the same time, light pressures that are generated at two focal points are made to act as rotational torque. Conventionally TIRF microscope is well known as the observation technology for the cell-membrane using the evanescent light as the exciting light. We can observe the local area images of the fluorescently labeled sugar chain that binds the glycoprotein. Using the proposed optical system, we can obtain the fluorescent distribution images on the cell-membrane.

Citation Download Citation

Kazuya Yamamoto, Ichirou Ishimaru, Yoshiki Fujii, Toshiki Yasokawa, Katsumi Ishizaki, Makoto Yoshida, Kaoru Takegawa, Naotaka Tanaka, Shigeki Kuriyama, Tsutomu Masaki, and Seiji Nakai "Imaging technology of three-dimensional distribution for sugar chain on single living cell membrane", Proc. SPIE 6374, Optomechatronic Actuators, Manipulation, and Systems Control, 63740L (19 October 2006); https://doi.org/10.1117/12.685893

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
9 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Glasses

Optical tweezers

Luminescence

3D image processing

Microscopy

Objectives

Microscopes

Show All Keywords

Keywords/Phrases

Search In:

Publication Years