17 October 2006 A fluorescent bio-aerosol point detector incorporating excitation, emission, and lifetime data
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Abstract
We present a novel approach to a biological point detector system: extracting maximal information from fluorescence by using as much of the full excitation-emission-lifetime (XML) fluorescence space as can be conveniently gathered. Our paper has two parts. In the first part, we present initial XML spectral data gathered under Phase I of the DARPA Spectral-Sensing of Bio-Aerosols (SSBA) program using a commercial laboratory spectrofluorometer and illustrate its analysis in a multi-dimensional Principal Components Analysis (PCA) data space. We demonstrate classification using the spectral angle (SA) methodology developed for hyperspectral imaging in this PCA hyperspace. In the second part, we present a design for a custom trigger sensor developed in Phase II of the DARPA program. This Phase II sensor was motivated by the Phase I results and is intended to exploit them by gathering XML data at a rate consistent with near-real-time triggering.
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Pierre C. Trepagnier, Pierre C. Trepagnier, Robert F. Dillon, Robert F. Dillon, Philip D. Henshaw, Philip D. Henshaw, David P. McCampbell, David P. McCampbell, } "A fluorescent bio-aerosol point detector incorporating excitation, emission, and lifetime data", Proc. SPIE 6377, Advanced Environmental, Chemical, and Biological Sensing Technologies IV, 637708 (17 October 2006); doi: 10.1117/12.684754; https://doi.org/10.1117/12.684754
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