25 October 2006 Analysis of oxytetracycline in water using a portable analyzer
Author Affiliations +
Abstract
Oxytetracycline (OTC) is extensively used in aquaculture worldwide for preventive and therapeutic purposes. Most of the drug, however, is discharged into the marine environment due to leaching from medicated feed and poor gastrointestinal (GI) absorption. Without exposure to sun light OTC has a long lifetime in the marine environment, therefore it is important to monitor and study its occurrence, distribution, fate and impact on the ecosystem. A portable tetracycline (TC) analyzer was developed in this laboratory for this purpose based on europium-sensitized luminescence. In this study, an assay method is developed for OTC analysis in water using this instrument. Water samples are filtered with glass wool; and solid phase extraction (SPE) is performed using Oasis HLB cartridges for OTC extraction and cleanup. Following reagent application, the samples are excited by 385 nm LED pulses; and time-resolved luminescence (TRL) is measured at 610 nm by a photomultiplier tube. A 0-3 ppm linear dynamic range (r2 = 0.9988) and a 0.021 ppb limit of detection were achieved with a typical <5% relative standard deviation.
© (2006) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Guoying Chen, Guoying Chen, Susan Braden, Susan Braden, Feng Qin, Feng Qin, } "Analysis of oxytetracycline in water using a portable analyzer", Proc. SPIE 6378, Chemical and Biological Sensors for Industrial and Environmental Monitoring II, 63780W (25 October 2006); doi: 10.1117/12.686401; https://doi.org/10.1117/12.686401
PROCEEDINGS
6 PAGES


SHARE
Back to Top