Advances in zinc sensors for studying zinc release events from pancreatic cells

Zeev Rosenzweig; Nitsa Rosenzweig; Georgeta Crivat

doi:10.1117/12.690653

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25 October 2006 Advances in zinc sensors for studying zinc release events from pancreatic cells

Zeev Rosenzweig, Nitsa Rosenzweig, Georgeta Crivat

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Proceedings Volume 6380, Smart Medical and Biomedical Sensor Technology IV; 638002 (2006) https://doi.org/10.1117/12.690653
Event: Optics East 2006, 2006, Boston, Massachusetts, United States

Abstract

This paper describes the fabrication and characterization of the analytical properties of fluorescence-based zinc ion sensing glass slides and antibody based zinc sensors and their application in monitoring zinc release from beta pancreatic cells. The zinc ion indicator ZnAF-2 {6-[N- [N', N'-bis (2-pyridinylmethyl)-2-aminoethyl] amino-3',6'-dihydroxy-spiro[isobenzofuran-1(3H),9'-[9H] xanthene]-3-one} was modified to include a sufficiently long linking aliphatic chain, with a terminal carboxyl functional group. The activated carboxyl-modified ZnAF-2 was conjugated to the amino silanized surface of glass slides and to free amino groups of the A2B5 antibody molecules. The sensors were used to monitor zinc ion release events from glucose-stimulated pancreatic cells.

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Zeev Rosenzweig, Nitsa Rosenzweig, and Georgeta Crivat "Advances in zinc sensors for studying zinc release events from pancreatic cells", Proc. SPIE 6380, Smart Medical and Biomedical Sensor Technology IV, 638002 (25 October 2006); https://doi.org/10.1117/12.690653

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