Cancer treatment often includes chemotherapy drugs that prevent cancer cell growth through a variety of biochemical
mechanisms, but are not target specific and kill other cells. Consequently, the dosage has a narrow range of safe and
effective use. Furthermore, because of the dangerous side-effects of these drugs, clinical trials are not performed, and
dosage is based on the limited statistics of the response of previously treated patients and administered according to body
surface area. Monitoring dosage during administration would clearly improve patient outcome. Unfortunately current
practices require 10-20 milliliters of blood per analysis, and multiple samples to profile pharmacokinetics may further jeopardize the patient's health. Saliva analysis has long been considered an attractive alternative, but the large sample
volumes are difficult to obtain. In an effort to overcome this limitation we have been investigating metal-doped sol-gels
to both separate drugs and their metabolites from saliva and generate surface-enhanced Raman spectra. We have
incorporated the sol-gel in a disposable pipette format, and generally no more than two drops (100 microL) of sample are
required to perform analysis. The detailed molecular vibrational information allows chemical identification, while the
increase in Raman scattering by six orders of magnitude or more allows detection of nanomolar concentrations.
Measurements of chemotherapy drugs at relevant concentration are presented.