Investigation of the excited states dynamics in the Chl d- containing cyanobacterium Acaryochloris marina by time and wavelength correlated single-photon counting

Franz-Josef Schmitt; Christoph Theiss; Karin Wache; Justus Fuesers; Stefan Andree; Andrianto Handojo; Anne Karradt; Daniela Kiekebusch; Hans Joachim Eichler; Hann-Jörg Eckert

doi:10.1117/12.689127

17 October 2006 Investigation of the excited states dynamics in the Chl d- containing cyanobacterium Acaryochloris marina by time and wavelength correlated single-photon counting

Franz-Josef Schmitt, Christoph Theiss, Karin Wache, Justus Fuesers, Stefan Andree, Andrianto Handojo, Anne Karradt, Daniela Kiekebusch, Hans Joachim Eichler, Hann-Jörg Eckert

Author Affiliations +

Proceedings Volume 6386, Optical Methods in the Life Sciences; 638607 (2006) https://doi.org/10.1117/12.689127
Event: Optics East 2006, 2006, Boston, Massachusetts, United States

Abstract

The phototrophic cyanobacterium Acaryochloris marina discovered in 1996 has a unique composition of the light harvesting system. The chlorophyll (Chl) antenna contains mainly Chl d instead of the usually dominant Chl a and the Phycobiliprotein (PBP) antenna has a simpler rod shaped structure than in typical cynobacteria [1]. The interaction of the photosynthetic subunits and especially the mechanisms regulating the energy transfer under different stress conditions are presently interesting and open fields in photosynthesis research. In this study we use time- and wavelength-resolved single photon counting to investigate the excited states dynamics in living cells of A.marina. The fluorescence dynamics is synchronistically monitored in the visible and near infrared spectrum with high signal to noise ratio and short data acquisition times while using low excitation light intensities. These attributes are necessary to investigate photosynthetic processes in sensitive biological samples, when the light emission varies due to metabolic changes. The results suggest a fast excitation energy transfer kinetics of 20-30 ps along the PBP antenna of A.marina followed by a transfer of about 60 ps to the Chl d antenna. Cells of A. marina which are stored at 0°C for some time show a decoupling of the PBP antenna, which is partially reversible when the sample is kept at 25 °C for a short time. Decoupling effects appearing after strong illumination with white light (1600 W/m²) suggest a mechanism which removes the PBP antenna at different stress conditions to avoid photo damage of the reaction center of Photosystem II (PS II).

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Franz-Josef Schmitt, Christoph Theiss, Karin Wache, Justus Fuesers, Stefan Andree, Andrianto Handojo, Anne Karradt, Daniela Kiekebusch, Hans Joachim Eichler, and Hann-Jörg Eckert "Investigation of the excited states dynamics in the Chl d- containing cyanobacterium Acaryochloris marina by time and wavelength correlated single-photon counting", Proc. SPIE 6386, Optical Methods in the Life Sciences, 638607 (17 October 2006); https://doi.org/10.1117/12.689127

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