13 October 2006 Fluorescence excitation-emission matrices of selected biological materials
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Abstract
EX-EM fluorescence matrices from suspensions of 11 vegetative bacteria, 5 spores, and 14 interferents (fungi, pollens) were measured and cross-sections of the selected bacteria were calculated. The changes of fluorescence characteristics of vegetative cells during their sporulation and starvation to death as well as spores during germination are shown. Influence of culture media on emission spectra and rate of spores formation under starving conditions was examined. Analysis of the measured fluorescence characteristics shows that double- or multi-wavelenghts excitation can make it possible to distinguish between particular groups of biological material, i.e., spores, vegetative cells, proteins, and interferents.
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M. Wlodarski, M. Wlodarski, M. Kaliszewski, M. Kaliszewski, M. Kwasny, M. Kwasny, K. Kopczynski, K. Kopczynski, Z. Zawadzki, Z. Zawadzki, Z. Mierczyk, Z. Mierczyk, J. Mlynczak, J. Mlynczak, E. Trafny, E. Trafny, M. Szpakowska, M. Szpakowska, } "Fluorescence excitation-emission matrices of selected biological materials", Proc. SPIE 6398, Optically Based Biological and Chemical Detection for Defence III, 639806 (13 October 2006); doi: 10.1117/12.687872; https://doi.org/10.1117/12.687872
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