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13 October 2006 Spectral detection of ultraviolet laser induced fluorescence from individual bio-aerosol particles
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Abstract
We present results of a measurement system designed for detecting the fluorescence spectrum of individual aerosol particles of biological warfare agents excited with laser pulses at wavelengths around 290 or 340 nm. The biological aerosol is prepared and directed into a narrow air beam. A red laser is focused on the aerosol beam and a trigger photomultiplier tube monitor the presence of individual particles by measuring the scattered light. When a particle is present in the detection volume, a laser pulse is triggered from an ultraviolet laser and the fluorescence spectrum is acquired with a spectrometer based on a diffraction grating and a 32 channels photomultiplier tube array with single-photon sensitivity. The spectrometer measures the fluorescence spectra in the wavelength region from 300 to 800 nm. In the experiment we used different simulants of biological warfare agents. These bioaerosol particles were excited by a commercial available gas laser (337 nm), or a laser (290 nm) that we have developed based on an optical parametric oscillator with intracavity sum-frequency mixing. In the analysis of the experiments we compare the measured signals (fluorescence spectra, total fluorescence energy and the scattered energy) from the individual bioaerosol particles excited with the two different ultraviolet wavelengths.
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Per Jonsson, Fredrik Kullander, Claes Vahlberg, Pär Jelger, Mikael Tiihonen, Pär Wästerby, Torbjörn Tjärnhage, and Mikael Lindgren "Spectral detection of ultraviolet laser induced fluorescence from individual bio-aerosol particles", Proc. SPIE 6398, Optically Based Biological and Chemical Detection for Defence III, 63980F (13 October 2006); https://doi.org/10.1117/12.689666
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