9 January 2007 A platform for the advanced spatial and temporal control of biomolecules
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Manipulating biomolecules at solid/liquid interfaces is important for the development of various biodevices including microarrays. Smart materials that enable both spatial and temporal control of biomolecules by combining switchability with patterned surface chemistry offer unprecedented levels of control of biomolecule manipulation. Such a system has been developed for the microscale spatial control over both DNA and cell growth on highly doped p-type silicon. Surface modification, involving plasma polymerisation of allylamine and poly(ethlylene glycol) grafting with subsequent laser ablation, led to the production of a patterned surface with dual biomolecule adsorption and desorption properties. On patterned surfaces, preferential electro-stimulated adsorption of DNA to the allylamine plasma polymer surface and subsequent desorption by the application of a negative bias was observed. The ability of this surface to control both DNA and cell attachment in four dimensions has been demonstrated, exemplifying its capacity to be used for complex biological studies such as gene function analysis. This system has been successfully applied to living microarray applications and is an exciting platform for any system incorporating biomolecules.
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Andrew L. Hook, Andrew L. Hook, Helmut Thissen, Helmut Thissen, Jason P. Hayes, Jason P. Hayes, Nicolas H. Voelcker, Nicolas H. Voelcker, "A platform for the advanced spatial and temporal control of biomolecules", Proc. SPIE 6413, Smart Materials IV, 64130C (9 January 2007); doi: 10.1117/12.695595; https://doi.org/10.1117/12.695595

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