14 December 2006 Encapsulation of cell into monodispersed hydrogels on microfluidic device
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Abstract
In here, we present the microfluidic approach to produce monodispersed microbeads that will contain viable cells. The utilization of microfludics is helpful to synthesize monodispersed alginate hydrogels and in situ encapsulate cell into the generating hydrogels in microfludic device. First, the condition of formation of hydrogels in multiphase flows including oil, CaCl2, and alginate was optimized. Based on the preliminary survey, microfludic device could easily manipulate the size of alginate beads having narrow size distribution. The microfluidic method manipulates the size of hydrogel microbeads from 30 to 200um with a variation less than 2%. For the proof of concept of cell entrapment, the live yeast expressing green fluorescence protein is successfully encapsulated in microfluidic device.
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Chang-Hyoung Choi, Chang-Hyoung Choi, Ji-Hye Lee, Ji-Hye Lee, Hyun-Woo Shim, Hyun-Woo Shim, Nae-Rym Lee, Nae-Rym Lee, Jae-Hoon Jung, Jae-Hoon Jung, Tae-Ho Yoon, Tae-Ho Yoon, Dong-Pyo Kim, Dong-Pyo Kim, Chang-Soo Lee, Chang-Soo Lee, } "Encapsulation of cell into monodispersed hydrogels on microfluidic device", Proc. SPIE 6416, Biomedical Applications of Micro- and Nanoengineering III, 641613 (14 December 2006); doi: 10.1117/12.708548; https://doi.org/10.1117/12.708548
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