23 March 2007 Quantitative intracellular calcium sensing with two-photon fluorescence lifetime imaging microscopy
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Abstract
In this work, we demonstrate the use of Two-Photon Fluorescence Lifetime Imaging Microscopy (TP-FLIM) for intracellular calcium ([Ca2+]i) measurement with a calcium sensitive fluorescent dye in a neuronal cell model and brain tissue. Calcium perturbations were induced via chemical stimulation and our results signify the potential of TP-FLIM for quantitative ([Ca2+]i) measurement within the physiological range of intracellular [Ca2+].
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Eddy Kuwana, Eddy Kuwana, Gracie Vargas-Adams, Gracie Vargas-Adams, Todd C. Pappas, Todd C. Pappas, Anton V. Liopo, Anton V. Liopo, Massoud Motamedi, Massoud Motamedi, } "Quantitative intracellular calcium sensing with two-photon fluorescence lifetime imaging microscopy", Proc. SPIE 6424, Photonic Therapeutics and Diagnostics III, 64242L (23 March 2007); doi: 10.1117/12.723959; https://doi.org/10.1117/12.723959
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