8 March 2007 Laser scanning confocal microscopy and laser tweezers based experiments to understand dentine-bacteria interactions
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Proceedings Volume 6425, Lasers in Dentistry XIII; 642504 (2007) https://doi.org/10.1117/12.701784
Event: SPIE BiOS, 2007, San Jose, California, United States
Abstract
Failure of endodontic treatment is commonly due to Enterococcal infection. In this study influence of chemical treatments of type-I collagen membrane by chemical agents commonly used in endodontic treatment on Enterococcus faecalis cell adherence was evaluated. In order to determine the change in number of adhering bacteria after chemical treatment, confocal laser scanning microscopy was used. For this, overnight culture of E faecalis in All Culture broth was applied to chemically treated type-I collagen membrane. It was found that Ca(OH)2 treated groups had statistically significant (p value=0.05) increase in population of bacteria adherence. The change in adhesion force between bacteria and collagen was determined by using optical tweezers (1064 nm). For this experiment, Type-I collagen membrane was soaked for 5 mins in a media that contained 50% all culture media and 50% saturated Ca(OH)2 . The membrane was spread on the coverslip, on which diluted bacterial suspension was added. The force of laser tweezers on the bacteria was estimated at different trap power levels using viscous drag method and trapping stiffness was calculated using Equipartition theorem method. Presence of Ca(OH)2 was found to increase the cell-substrate adherence force from 0.38pN to >2.1pN. Together, these experiments show that it was highly probable that the increase in adherence to collagen was due to a stronger adhesion in the presence of Ca (OH)2.
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Sum Chee Peng, Sum Chee Peng, Samarendra Mohanty, Samarendra Mohanty, P. K. Gupta, P. K. Gupta, Anil Kishen, Anil Kishen, } "Laser scanning confocal microscopy and laser tweezers based experiments to understand dentine-bacteria interactions", Proc. SPIE 6425, Lasers in Dentistry XIII, 642504 (8 March 2007); doi: 10.1117/12.701784; https://doi.org/10.1117/12.701784
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