Demonstration of structural alterations in experimental corneal infectious model using multiphoton microscopy

Wen Lo; Hsin-Yuan Tan; Yuh-Ling Chang; Yen Sun; Sung-Jan Lin; Shiou-Hwa Jee; Chen-Yuan Dong

doi:10.1117/12.699468

26 March 2007 Demonstration of structural alterations in experimental corneal infectious model using multiphoton microscopy

Wen Lo, Hsin-Yuan Tan, Yuh-Ling Chang, Yen Sun, Sung-Jan Lin, Shiou-Hwa Jee, Chen-Yuan Dong

Author Affiliations +

Proceedings Volume 6426, Ophthalmic Technologies XVII; 64260Y (2007) https://doi.org/10.1117/12.699468
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

The aim of this study is to assess the application of multiphoton autofluorescence and second harmonic generation (SHG) microscopy for investigating the structural alterations and the pattern of microbial spreading during corneal infectious process in an in vitro organ culture model. The autofluorescence spectrum derived from pathogens allows us to monitoring the pattern of microbial spreading within corneal lamellae. In addition, the destruction and regeneration of second harmonic generating collagen during infectious process can also be monitored in a non-invasive fashion. Therefore we propose that multiphoton microscopy may potentially be applied as an effective monitoring tool for corneal infection studies.

Citation Download Citation

Wen Lo, Hsin-Yuan Tan, Yuh-Ling Chang, Yen Sun, Sung-Jan Lin, Shiou-Hwa Jee, and Chen-Yuan Dong "Demonstration of structural alterations in experimental corneal infectious model using multiphoton microscopy", Proc. SPIE 6426, Ophthalmic Technologies XVII, 64260Y (26 March 2007); https://doi.org/10.1117/12.699468

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