26 March 2007 Demonstration of structural alterations in experimental corneal infectious model using multiphoton microscopy
Author Affiliations +
Proceedings Volume 6426, Ophthalmic Technologies XVII; 64260Y (2007) https://doi.org/10.1117/12.699468
Event: SPIE BiOS, 2007, San Jose, California, United States
Abstract
The aim of this study is to assess the application of multiphoton autofluorescence and second harmonic generation (SHG) microscopy for investigating the structural alterations and the pattern of microbial spreading during corneal infectious process in an in vitro organ culture model. The autofluorescence spectrum derived from pathogens allows us to monitoring the pattern of microbial spreading within corneal lamellae. In addition, the destruction and regeneration of second harmonic generating collagen during infectious process can also be monitored in a non-invasive fashion. Therefore we propose that multiphoton microscopy may potentially be applied as an effective monitoring tool for corneal infection studies.
© (2007) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Wen Lo, Wen Lo, Hsin-Yuan Tan, Hsin-Yuan Tan, Yuh-Ling Chang, Yuh-Ling Chang, Yen Sun, Yen Sun, Sung-Jan Lin, Sung-Jan Lin, Shiou-Hwa Jee, Shiou-Hwa Jee, Chen-Yuan Dong, Chen-Yuan Dong, } "Demonstration of structural alterations in experimental corneal infectious model using multiphoton microscopy", Proc. SPIE 6426, Ophthalmic Technologies XVII, 64260Y (26 March 2007); doi: 10.1117/12.699468; https://doi.org/10.1117/12.699468
PROCEEDINGS
8 PAGES


SHARE
Back to Top