5 March 2007 Microscopic analysis of structural changes in diode-laser-welded corneal stroma
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Proceedings Volume 6426, Ophthalmic Technologies XVII; 642614 (2007) https://doi.org/10.1117/12.700285
Event: SPIE BiOS, 2007, San Jose, California, United States
Abstract
Diode laser welding of the cornea is a technique used to provide immediate sealing of corneal wounds: the cut is stained with a water solution of Indocyanine Green and is then irradiated with an 810 nm laser at low power densities (12-16 W/cm2), which induces a localized heating of the stroma in the range 55-66 °C range. In this study, we present a microscopic analysis aimed at evaluating the structural modifications induced in the stromal collagen of pig eyes during the laser welding of corneal wounds. Cornea samples obtained from twenty freshly-enucleated eyes were cut with a pre-calibrated knife and subjected to the laser welding procedure. Histological slices of the laser-welded stroma were examined by means of optical and transmission electron microscopy. These analyses indicated that bridges of lamellar structures crossed the wound edges with no presence of a coagulation effect. After laser welding, collagen fibrils appeared differently oriented among themselves in comparison with those of the control samples, but with similar mean fibril diameters. The laser-induced effect appeared to be confined to the ICG stained area of the cut walls, and no heat damage was observed at the operative power levels of laser corneal welding.
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Paolo Matteini, Paolo Matteini, Francesca Rossi, Francesca Rossi, Luca Menabuoni, Luca Menabuoni, Roberto Pini, Roberto Pini, } "Microscopic analysis of structural changes in diode-laser-welded corneal stroma", Proc. SPIE 6426, Ophthalmic Technologies XVII, 642614 (5 March 2007); doi: 10.1117/12.700285; https://doi.org/10.1117/12.700285
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