In-vitro visualization of corneal wound healing in an organ culture model using multiphoton autofluorescence and second harmonic generation microscopy

Wen Lo; Yuh-Ling Chang; Yen Sun; Sung-Jan Lin; Shiou-Hwa Jee; Hsin-Yuan Tan; Chen-Yuan Dong

doi:10.1117/12.699709

26 March 2007 In-vitro visualization of corneal wound healing in an organ culture model using multiphoton autofluorescence and second harmonic generation microscopy

Wen Lo, Yuh-Ling Chang, Yen Sun, Sung-Jan Lin, Shiou-Hwa Jee, Hsin-Yuan Tan, Chen-Yuan Dong

Author Affiliations +

Proceedings Volume 6426, Ophthalmic Technologies XVII; 642617 (2007) https://doi.org/10.1117/12.699709
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

The aim of this work is to image the wound healing process of cornea in an in vitro organ culture model with noninvasive multiphoton imaging modality. Autofluorescence and second harmonic generation (SHG) were respectively used to monitor the alterations of cellular and collagenous components during wound healing processes. Within additional developments, this approach may be applied to in vivo visualization of corneal structural destruction and the subsequent regeneration.

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Wen Lo, Yuh-Ling Chang, Yen Sun, Sung-Jan Lin, Shiou-Hwa Jee, Hsin-Yuan Tan, and Chen-Yuan Dong "In-vitro visualization of corneal wound healing in an organ culture model using multiphoton autofluorescence and second harmonic generation microscopy", Proc. SPIE 6426, Ophthalmic Technologies XVII, 642617 (26 March 2007); https://doi.org/10.1117/12.699709

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