Improved murine glioma detection following modified diet and photobleaching of skin PpIX fluorescence

Summer L. Gibbs; Julia A. O'Hara; P. Jack Hoopes; Brian W. Pogue

doi:10.1117/12.700456

27 February 2007 Improved murine glioma detection following modified diet and photobleaching of skin PpIX fluorescence

Summer L. Gibbs, Julia A. O'Hara, P. Jack Hoopes, Brian W. Pogue

Proceedings Volume 6427, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVI; 64270D (2007) https://doi.org/10.1117/12.700456
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

The Aminolevulinic Acid (ALA) - Protoporphyrin IX (PpIX) system is unique in the world of photosensitizers in that the prodrug ALA is enzymatically transformed via the tissue of interest into fluorescently detectable levels of PpIX. This system can be used to monitor cellular metabolism of tumor tissue for applications such as therapy monitoring. Detecting PpIX fluorescence noninvasively has proven difficult due to the high levels of PpIX produced in the skin compared to other tissue both with and without ALA administration. In the current study, methods to decrease skin PpIX autofluorescence and skin PpIX fluorescence following ALA administration have been examined. Use of a purified diet is found to decrease both skin PpIX autofluorescence and skin PpIX fluorescence following ALA administration, while addition of a broad spectrum antibiotic to the water shows little effect. Following ALA administration, improved brain tumor detection is seen when skin PpIX fluorescence is photobleached via blue light prior to transmission spectroscopic measurements of tumor bearing and control animals. Both of these methods to decrease skin PpIX autofluorescence and skin PpIX fluorescence following ALA administration are shown to have a large effect on the ability to detect tumor tissue PpIX fluorescence noninvasively in vivo.

Citation Download Citation

Summer L. Gibbs, Julia A. O'Hara, P. Jack Hoopes, and Brian W. Pogue "Improved murine glioma detection following modified diet and photobleaching of skin PpIX fluorescence", Proc. SPIE 6427, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVI, 64270D (27 February 2007); https://doi.org/10.1117/12.700456

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KEYWORDS

Luminescence

Skin

Tumors

Tissues

Brain

Spectroscopy

Head

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