20 February 2007 Optical scatter imaging: a microscopic modality for the rapid morphological assay of living cells
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Proceedings Volume 6431, Multimodal Biomedical Imaging II; 643102 (2007); doi: 10.1117/12.702247
Event: SPIE BiOS, 2007, San Jose, California, United States
Abstract
Tumors derived from epithelial cells comprise the majority of human tumors and their growth results from the accumulation of multiple mutations affecting cellular processes critical for tissue homeostasis, including cell proliferation and cell death. To understand these processes and address the complexity of cancer cell function, multiple cellular responses to different experimental conditions and specific genetic mutations must be analyzed. Fundamental to this endeavor is the development of rapid cellular assays in genetically defined cells, and in particular, the development of optical imaging methods that allow dynamic observation and real-time monitoring of cellular processes. In this context, we are developing an optical scatter imaging technology that is intended to bridge the gap between light and electron microscopy by rapidly providing morphometric information about the relative size and shape of non-spherical organelles, with sub-wavelength resolution. Our goal is to complement current microscopy techniques used to study cells in-vitro, especially in long-term time-lapse studies of living cells, where exogenous labels can be toxic, and electron microscopy will destroy the sample. The optical measurements are based on Fourier spatial filtering in a standard microscope, and could ultimately be incorporated into existing high-throughput diagnostic platforms for cancer cell research and histopathology of neoplastic tissue arrays. Using an engineered epithelial cell model of tumor formation, we are currently studying how organelle structure and function are altered by defined genetic mutations affecting the propensity for cell death and oncogenic potential, and by environmental conditions promoting tumor growth. This talk will describe our optical scatter imaging technology and present results from our studies on apoptosis, and the function of BCL-2 family proteins.
© (2007) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Nada N. Boustany, "Optical scatter imaging: a microscopic modality for the rapid morphological assay of living cells", Proc. SPIE 6431, Multimodal Biomedical Imaging II, 643102 (20 February 2007); doi: 10.1117/12.702247; https://doi.org/10.1117/12.702247
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KEYWORDS
Cell death

Particles

Calcium

Optical imaging

Tissue optics

Genetics

Electron microscopy

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