Modeling and image reconstruction in spectrally resolved bioluminescence tomography

Hamid Dehghani; Brian W. Pogue; Scott C. Davis; Michael S. Patterson

doi:10.1117/12.698931

13 February 2007 Modeling and image reconstruction in spectrally resolved bioluminescence tomography

Hamid Dehghani, Brian W. Pogue, Scott C. Davis, Michael S. Patterson

Proceedings Volume 6434, Optical Tomography and Spectroscopy of Tissue VII; 64340V (2007) https://doi.org/10.1117/12.698931
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Recent interest in modeling and reconstruction algorithms for Bioluminescence Tomography (BLT) has increased and led to the general consensus that non-spectrally resolved intensity-based BLT results in a non-unique problem. However, the light emitted from, for example firefly Luciferase, is widely distributed over the band of wavelengths from 500 nm to 650 nm and above, with the dominant fraction emitted from tissue being above 550 nm. This paper demonstrates the development of an algorithm used for multi-wavelength 3D spectrally resolved BLT image reconstruction in a mouse model. It is shown that using a single view data, bioluminescence sources of up to 15 mm deep can be successfully recovered given correct information about the underlying tissue absorption and scatter.

Citation Download Citation

Hamid Dehghani, Brian W. Pogue, Scott C. Davis, and Michael S. Patterson "Modeling and image reconstruction in spectrally resolved bioluminescence tomography", Proc. SPIE 6434, Optical Tomography and Spectroscopy of Tissue VII, 64340V (13 February 2007); https://doi.org/10.1117/12.698931

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