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21 February 2007 An in vitro model for retinal laser damage
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Abstract
Ocular laser exposures resulting in damage at the retina typically involve cellular alterations in the retinal pigment epithelial (RPE) layer. To provide guidelines for eye-safe exposure to lasers, the laser safety community has relied on damage assessment in nonhuman primate studies. Simple and reliable model systems for laser bioeffects that use cultured RPE cells, rather than animals, are thus desirable. We have characterized our artificially pigmented hTERT-RPE1 model by identifying ED50 thresholds over a wide range of laser parameters and cell culture conditions. When summarized as action spectra and temporal action profiles (log threshold fluence versus log exposure duration), trends (pigment-dependent) in our cell model data are strikingly similar to the threshold trends reported for animal models (literature). In addition, the rapidity and flexibility (laser delivery) with which studies are performed in our culture model has benefited computational modeling efforts.
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Michael L. Denton, Michael S. Foltz, Kurt J. Schuster, Larry E. Estlack, Harvey M. Hodnett, Gary D. Noojin, and Robert J. Thomas "An in vitro model for retinal laser damage", Proc. SPIE 6435, Optical Interactions with Tissue and Cells XVIII, 643515 (21 February 2007); https://doi.org/10.1117/12.701067
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