7 February 2007 In vivo dynamic light scattering measurements of red blood cell aggregation
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The tendency of red blood cells (RBCs) to aggregate is considered to be a critical biological activity, which contributes to blood viscosity. The ability to assess blood viscosity parameters non-invasively can play an important role in a variety of fields of medicine. Toward achieving this goal, we have attempted to follow the kinetics of red blood cell's aggregation process non-invasively. In this work, we have generated the optical signals of RBC aggregation and have studied them both in vitro and in vivo utilizing a dynamic light scattering (DLS) approach. The system was built and calibrated, first in vitro by using micro-sphere suspensions of known particle sizes and subsequently in vitro, on a sample of whole blood. Time dependent behavior of a function expressed in terms of autocorrelation of light intensity fluctuations was analyzed. The correspondence between in vitro aggregation tests and in vivo results was revealed and is explained in terms of modified diffusion theory and WKB approximation for light scattering mediated by aggregates. Therefore, we have demonstrated the applicability of a DLS based technique for the non-invasive measurement of blood particle sizes and blood viscosity.
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Ilya Fine, Ilya Fine, Alexander Kaminsky, Alexander Kaminsky, } "In vivo dynamic light scattering measurements of red blood cell aggregation", Proc. SPIE 6436, Complex Dynamics and Fluctuations in Biomedical Photonics IV, 64360C (7 February 2007); doi: 10.1117/12.699503; https://doi.org/10.1117/12.699503

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