In order to discuss the relative factors affecting the optical clearing effect of agents on skin tissues, six
hydroxy-terminated and saturated alcohols with different refractive index and molecular weight were chosen as the
optical clearing agents (OCAs). After being treated by different OCAs, the change of transmitted intensity of porcine
skins in vitro was measured by single integrating sphere system. The results showed the optical clearing effects of six
OCAs, i.e., glycerol, PEG400, PEG200, 1,3-propylene glycol, 1,4-butanediol and 1-butanol, arranged in the descending
order. Based on the above results, the refractive index and molecular weight was further discussed. The optical clearing
effect of alcohols has been deduced to have negative correlation with refractive index (r=-0.608), but no correlation with
molecular weight (r= 0.008).