19 February 2007 Confocal time-resolved fluorescence anisotropy imaging
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A confocal time-resolved fluorescence anisotropy imaging set-up is presented. It combines a confocal laser scanning microscope equipped with a pulsed laser and two time gated detection systems with 4 gates each (LiMo, originally developed for FLIM). The anisotropy decays obtained with the time gating system yield results that compare well with the high time-resolution (non-imaging) decays recorded using Time Correlated Single Photon Counting. Time resolved anisotropy imaging experiments on cells expressing GPI-GFP were carried out. Clear distinction could be made between the anisotropy in the plasma membrane and in the interior of the cell.
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Arjen N. Bader, Erik G. Hofman, Paul van Bergen en Henegouwen, Hans C. Gerritsen, "Confocal time-resolved fluorescence anisotropy imaging", Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64410C (19 February 2007); doi: 10.1117/12.702031; https://doi.org/10.1117/12.702031

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