19 February 2007 Epidermal growth factor signaling studied using multidimensional single molecule fluorescence microscopy
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Abstract
Quantification of the oligomerisation state and conformational changes of the epidermal growth factor receptor in its constitutive and active states are critical to fully understand their signal transduction pathway. We show the ability of single-molecule fluorescence microscopy to determine this information and its implementation by combining multidimensional emission optics with total internal reflection fluorescence microscopy. Importantly, the signalling process is analysed in live cells under physiological conditions.
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Stephen E. D. Webb, Selene K. Roberts, Sarah R. Needham, Daniel J. Rolfe, Chris J. Tynan, Marisa Martin-Fernandez, "Epidermal growth factor signaling studied using multidimensional single molecule fluorescence microscopy", Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64410E (19 February 2007); doi: 10.1117/12.701417; https://doi.org/10.1117/12.701417
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