Semiconductor light-emitting devices with in-built bioreaction chambers

H. D. Summers; D. R. Matthews; R. J. Errington; K. L. Njoh; P. J. Smith; A. D. Goater; J. P. H. Burt; N. H. Rizvi; A. Menachery; D. J. Morris

doi:10.1117/12.700411

19 February 2007 Semiconductor light-emitting devices with in-built bioreaction chambers

H. D. Summers, D. R. Matthews, R. J. Errington, K. L. Njoh, P. J. Smith, A. D. Goater, J. P. H. Burt, N. H. Rizvi, A. Menachery, D. J. Morris

Author Affiliations +

Proceedings Volume 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V; 64410Y (2007) https://doi.org/10.1117/12.700411
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

We demonstrate complete integration of a fluorescence-based assay in that the analyte well is also an optical emitter. Laser machining is used to create 'active micro-wells' within semiconductor light emitting diode and laser structures. These are then used to optically excite fluorescently-labelled beads in solution within the well. The results show efficient illumination on a par with traditional lamp-based excitation. This technology therefore provides active microwell plates with completely localized excitation, confined to the analysis well, that can be engineered via the micro-well geometry. The micro-wells have also been machined within the cavity of lasing semiconductor structures and coherent emission maintained. Thus lasing multi-well plates are also realizable.

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H. D. Summers, D. R. Matthews, R. J. Errington, K. L. Njoh, P. J. Smith, A. D. Goater, J. P. H. Burt, N. H. Rizvi, A. Menachery, and D. J. Morris "Semiconductor light-emitting devices with in-built bioreaction chambers", Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64410Y (19 February 2007); https://doi.org/10.1117/12.700411

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