Precise microinjection into living cells by summation of fluorescence intensity

Kiyoshi Taninaka; A. Yabuki; A. Ito; T. Harada

doi:10.1117/12.704741

19 February 2007 Precise microinjection into living cells by summation of fluorescence intensity

Kiyoshi Taninaka, A. Yabuki, A. Ito, T. Harada

Proceedings Volume 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V; 64410Z (2007) https://doi.org/10.1117/12.704741
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

It is difficult to introduce a specific amount of a substance into cells by existing injection methods because there is no appropriate method of directly measuring the quantity of the injected substance. Although radioisotopes can be used, there is currently no apparatus that can practically handle such radioisotopes. The measurement of the diameter of a liquid droplet in air or oil is affected by surface tension if the liquid droplet is very small; but this issue does not occur with microinjection, in which a water solution is discharged under pressure through a capillary and into a cell. It is also difficult to measure the density or mass of the injected substance because of the low discharge rate, unlike the case of inkjet printers. To solve these problems, we propose a method of precise microinjection by summation of fluorescence intensity. In addition, we developed a new pressure pulse injection device that generates pressure with a rectangular waveform and a precise amplitude and pulse width to improve controllability of the discharge amount. Lastly, when the above device and method are combined, the coefficient of correlation between the specified number of pressure pulses per unit of time and the actual discharge amount exceeded 0.999. This research paper describes in detail the measurement system, standalone performance, and quantities of substances introduced into living cells.

Citation Download Citation

Kiyoshi Taninaka, A. Yabuki, A. Ito, and T. Harada "Precise microinjection into living cells by summation of fluorescence intensity", Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64410Z (19 February 2007); https://doi.org/10.1117/12.704741

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KEYWORDS

Luminescence

Liquids

Capillaries

Calibration

Protactinium

Radioisotopes

Absorption

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