A high-resolution optofluidic microscope with optical tweezer actuation

Xin Heng; Edward Hsiao; Demetri Psaltis; Changhuei Yang

doi:10.1117/12.701728

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19 February 2007 A high-resolution optofluidic microscope with optical tweezer actuation

Xin Heng, Edward Hsiao, Demetri Psaltis, Changhuei Yang

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Proceedings Volume 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V; 644113 (2007) https://doi.org/10.1117/12.701728
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

In this paper, we will report our recent development of a new type of OptoFluidic Microscope (OFM) that is capable of delivering resolution beyond the diffraction limit of light. Accurate control of the sample translation is accomplished by adopting an optical tweezer scanner into the system. During the image acquisition, a two-dimensional nanoaperture array defined on a thin aluminum film acts as an array of ultra-fine illumination sources. The imaging system is tested and demonstrated by using polystyrene beads and green algae (Chlamydomonas). Properties of the system are reported and discussed.

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Xin Heng, Edward Hsiao, Demetri Psaltis, and Changhuei Yang "A high-resolution optofluidic microscope with optical tweezer actuation", Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 644113 (19 February 2007); https://doi.org/10.1117/12.701728

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