19 February 2007 Axially resolved cell imaging by intensity modulated total internal reflection fluorescence microscopy (IM-TIRFM)
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Abstract
Intensity modulation was introduced to total internal reflection fluorescence microscopy (TIRFM). This permits to obtain information about the axial distribution of fluorescent dyes in close vicinity to the surface of adherent cells and allows the application of (commercial) objective based TIRFM systems at a fixed angle of illumination. However, this new method seems to be less suitable for quantitative measurements of cell-substrate topology as compared to variableangle prism based TIRFM.
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Herbert Schneckenburger, Herbert Schneckenburger, Harald Baumann, Harald Baumann, Michael Wagner, Michael Wagner, Wolfgang S. L. Strauss, Wolfgang S. L. Strauss, } "Axially resolved cell imaging by intensity modulated total internal reflection fluorescence microscopy (IM-TIRFM)", Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64411E (19 February 2007); doi: 10.1117/12.699826; https://doi.org/10.1117/12.699826
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