Changes of delayed luminescence and CLSM imaging of chlorophyll autofluorescence during petal development in Gerbera hybrida

Wen Li Chen D.D.S.; YuHua Bi; Qiang Li; Quan Zhou; Da Xing

doi:10.1117/12.717026

19 February 2007 Changes of delayed luminescence and CLSM imaging of chlorophyll autofluorescence during petal development in Gerbera hybrida

Wen Li Chen D.D.S., YuHua Bi, Qiang Li, Quan Zhou, Da Xing

Author Affiliations +

Proceedings Volume 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V; 64411X (2007) https://doi.org/10.1117/12.717026
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Gerbera hybrida (Shenzhen No.5) seedlings' inflorescence development was divided into six stages (P1-P6). With these six stages petal, delayed luminescence (DL) were observed during petal development using lab-made detector system, fluorescence spectrum and confocal imaging were also observed. The results showed that the intensity of DL were increased during P1-P4 and decreased in P5 and P6; with the excitation wavelength of 488 nm, fluorescence spectra were obviously different during P1-P6 stages; imaging of chlorophyll autofluorescence by confocal laser scanning microscopy (CLSM) showed that the intensity were stronger in P3 than in P1, while P6 stage autofluorescence only displayed in guard cell of epidermis. Our results suggested that the DL technique, combining with fluorescence spectra and CLSM imaging, might be useful for the rapid and noninvasive evaluation of chlorophyll content and degradation in petal development in Gerbera hybrida.

Citation Download Citation

Wen Li Chen D.D.S., YuHua Bi, Qiang Li, Quan Zhou, and Da Xing "Changes of delayed luminescence and CLSM imaging of chlorophyll autofluorescence during petal development in Gerbera hybrida", Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64411X (19 February 2007); https://doi.org/10.1117/12.717026

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