10 February 2007 Clinical in vivo two-photon microendoscopy for intradermal high-resolution imaging with GRIN optics
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Multiphoton tomography with the clinical femtosecond laser system DermaInspect has become an important non-invasive high resolution imaging tool for skin research, melanoma detection, and in situ drug monitoring of pharmaceutical and cosmetical components. The detection of endogenous fluorophores and SHG active biostructures such as mitochondrial NAD(P)H, melanin in melancytes and basal cells, as well as the extracellular matrix components elastin and collagen is achieved with submicron resolution when using high NA focusing optics. So far, the working distance was limited to 200 µm. In addition, the focusing optics was large in diameter (2-3 cm). Here we report for the first time on clinical deep-tissue high-resolution imaging with a novel high NA rigid GRIN microendoscope which extends the potential of clinical multiphoton tomography significantly. We performed the very first clinical in vivo measurements with two-photon endoscopes and studied wounds of patients with ulcus cruris.
© (2007) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Karsten König, Karsten König, Alexander Ehlers, Alexander Ehlers, Iris Riemann, Iris Riemann, Selma Schenkl, Selma Schenkl, Bernhard Messerschmidt, Bernhard Messerschmidt, Rainer Bückle, Rainer Bückle, Ronan Le Harzic, Ronan Le Harzic, Peter Elsner, Peter Elsner, Martin Kaatz, Martin Kaatz, } "Clinical in vivo two-photon microendoscopy for intradermal high-resolution imaging with GRIN optics", Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 644215 (10 February 2007); doi: 10.1117/12.702380; https://doi.org/10.1117/12.702380


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