10 February 2007 Clinical in vivo two-photon microendoscopy for intradermal high-resolution imaging with GRIN optics
Author Affiliations +
Proceedings Volume 6442, Multiphoton Microscopy in the Biomedical Sciences VII; 644215 (2007); doi: 10.1117/12.702380
Event: SPIE BiOS, 2007, San Jose, California, United States
Abstract
Multiphoton tomography with the clinical femtosecond laser system DermaInspect has become an important non-invasive high resolution imaging tool for skin research, melanoma detection, and in situ drug monitoring of pharmaceutical and cosmetical components. The detection of endogenous fluorophores and SHG active biostructures such as mitochondrial NAD(P)H, melanin in melancytes and basal cells, as well as the extracellular matrix components elastin and collagen is achieved with submicron resolution when using high NA focusing optics. So far, the working distance was limited to 200 µm. In addition, the focusing optics was large in diameter (2-3 cm). Here we report for the first time on clinical deep-tissue high-resolution imaging with a novel high NA rigid GRIN microendoscope which extends the potential of clinical multiphoton tomography significantly. We performed the very first clinical in vivo measurements with two-photon endoscopes and studied wounds of patients with ulcus cruris.
© (2007) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Karsten König, Alexander Ehlers, Iris Riemann, Selma Schenkl, Bernhard Messerschmidt, Rainer Bückle, Ronan Le Harzic, Peter Elsner, Martin Kaatz, "Clinical in vivo two-photon microendoscopy for intradermal high-resolution imaging with GRIN optics", Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 644215 (10 February 2007); doi: 10.1117/12.702380; https://doi.org/10.1117/12.702380
PROCEEDINGS
6 PAGES


SHARE
KEYWORDS
GRIN lenses

Collagen

In vivo imaging

Skin

Endoscopes

Femtosecond phenomena

Imaging systems

RELATED CONTENT

In vivo multiphoton tomography in skin aging studies
Proceedings of SPIE (February 19 2009)
Multiphoton tomography of epidermis and dermis
Proceedings of SPIE (March 30 2005)
Multiphoton tomography for tissue engineering
Proceedings of SPIE (February 06 2008)
In-vivo multiphoton-endoscopy of endogenous skin fluorophores
Proceedings of SPIE (February 10 2007)
Current developments in clinical multiphoton tomography
Proceedings of SPIE (February 26 2010)

Back to Top