10 February 2007 Study of skeletal muscle cross-bridge population dynamics by second harmonic generation
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The high degree of structural order in skeletal muscle allows imaging of this tissue by Second Harmonic Generation (SHG). Biochemical and colocalization studies have gathered an increasing wealth of clues for the attribution of the molecular origin of the muscle SHG signal to the motor protein myosin. Thus, SHG represents a potentially very powerful tool in the investigation of structural dynamics occurring in muscle during active production of force and/or shortening. A full characterization of the polarization-dependence of the SHG signal represents a very selective information on the orientation of the emitting proteins and their dynamics during contraction, provided that different physiological states of muscle (relaxed, rigor and active) exhibit distinct patterns of SHG polarization dependence. Here polarization data are obtained from single frog muscle fibers at rest and during isometric contraction and interpreted, by means of a model, in terms of an average orientation of the SHG emitters which are structured with a cylindrical symmetry about the fiber axis. The setup is optimized for accurate polarization measurements with SHG, combined with a line scan imaging method allowing acquisition of SHG polarization curves in different physiological states. We demonstrate that muscle fiber displays a measurable variation of the orientation of SHG emitters with the transition from rest to isometric contraction.
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Valentina Nucciotti, C. Stringari, L. Sacconi, F. Vanzi, C. Tesi, N. Pirrodi, C. Poggesi, C. Castiglioni, A. Milani, M. Linari, G. Piazzesi, V. Lombardi, F. S. Pavone, "Study of skeletal muscle cross-bridge population dynamics by second harmonic generation", Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 644219 (10 February 2007); doi: 10.1117/12.700419; https://doi.org/10.1117/12.700419

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