10 February 2007 In vivo multiphoton endoscopy of endogenous skin fluorophores
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Proceedings Volume 6442, Multiphoton Microscopy in the Biomedical Sciences VII; 64421Y (2007); doi: 10.1117/12.702394
Event: SPIE BiOS, 2007, San Jose, California, United States
Abstract
Multiphoton tomography offers a painless method to examine patients under natural physiological conditions in vivo. Multiphoton excitation induces a weak autofluorescence of naturally endogenous fluorescent bio-molecules, such as flavines, NAD(P)H, metal-free porphyrines, components of lipofuscin, elastin and keratin. Additionally, collagen can be detected by second harmonic generation (SHG). Due to the nonlinearity, the effects occur only in a very tight focus, where the photon density is high enough. This leads to high axial and lateral resolution of <1μm without any need of a confocal detection and avoids out-of-focus damage. The limited depth range, given by the working distance of the focusing optics, is overcome with a gradient index-lens (GRIN-lens) based endoscope. In this work we present the first results of clinical applications in vivo of gradient-index lens endoscopes. Images of e.g. elastin and collagen (SHG) in the dermal layer of human skin are presented.
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Alexander Ehlers, Selma Schenkl, Iris Riemann, Bernhard Messerschmidt, Martin Kaatz, Rainer Bückle, Karsten König, "In vivo multiphoton endoscopy of endogenous skin fluorophores", Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 64421Y (10 February 2007); doi: 10.1117/12.702394; https://doi.org/10.1117/12.702394
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