14 February 2007 Influence of the surface hydrophobicity on fluorescence correlation spectroscopy measurements
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Fluorescence correlation spectroscopy (FCS) is a powerful experimental technique used to analyze the diffusion at the single molecule level in solution. FCS is based on the temporal autocorrelation of fluorescent signal generated by dye molecules diffusing through a small confocal volume. These measurements are mostly carried out in a chambered coverglass, close to the glass substrate. In this report, we discuss how the chemical nature of the glass-water interface may interact with the free diffusion of molecules. Our results reveal a strong influence, up to a few μm from the interface, of the surface hydrophobicity degree. This influence is assessed through the relative weight of the two dimension diffusion process observed at the vicinity of the surface.
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Céline Boutin, Céline Boutin, Rodolphe Jaffiol, Rodolphe Jaffiol, Jérome Plain, Jérome Plain, Pascal Royer, Pascal Royer, "Influence of the surface hydrophobicity on fluorescence correlation spectroscopy measurements", Proc. SPIE 6444, Ultrasensitive and Single-Molecule Detection Technologies II, 64440J (14 February 2007); doi: 10.1117/12.698889; https://doi.org/10.1117/12.698889

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