An Optical Approach for Non-invasive Blood Clot Testing

Vyacheslav Kalchenko; Alexander Brill; Ilya Fine; Alon Harmelin

doi:10.1117/12.699491

14 February 2007 An optical approach for non-invasive blood clot testing

Vyacheslav Kalchenko, Alexander Brill, Ilya Fine, Alon Harmelin

Proceedings Volume 6445, Optical Diagnostics and Sensing VII; 644508 (2007) https://doi.org/10.1117/12.699491
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Physiological blood coagulation is an essential biological process. Current tests for plasma coagulation (clotting) need to be performed ex vivo and require fresh blood sampling for every test. A recently published work describes a new, noninvasive, in vivo approach to assess blood coagulation status during mechanical occlusion¹. For this purpose, we have tested this approach and applied a controlled laser beam to blood micro-vessels of the mouse ear during mechanical occlusion. Standard setup for intravital transillumination videomicroscopy and laser based imaging techniques were used for monitoring the blood clotting process. Temporal mechanical occlusion of blood vessels in the observed area was applied to ensure blood flow cessation. Subsequently, laser irradiation was used to induce vascular micro-injury. Changes in the vessel wall, as well as in the pattern of blood flow, predispose the area to vascular thrombosis, according to the paradigm of Virchow's triad. In our experiments, two elements of Virchow's triad were used to induce the process of clotting in vivo, and to assess it optically. We identified several parameters that can serve as markers of the blood clotting process in vivo. These include changes in light absorption in the area of illumination, as well as changes in the pattern of the red blood cells' micro-movement in the vessels where blood flow is completely arrested. Thus, our results indicate that blood coagulation status can be characterized by non-invasive, in vivo methodologies.

Citation Download Citation

Vyacheslav Kalchenko, Alexander Brill, Ilya Fine, and Alon Harmelin "An optical approach for non-invasive blood clot testing", Proc. SPIE 6445, Optical Diagnostics and Sensing VII, 644508 (14 February 2007); https://doi.org/10.1117/12.699491

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Blood

Injuries

Blood circulation

In vivo imaging

Blood vessels

Ear

Animal model studies

Show All Keywords

Keywords/Phrases

Search In:

Publication Years