Investigation of the operating mechanism of a diffraction-based sensor

J. Valiani; Matthew F. Paige

doi:10.1117/12.698414

Abstract

In this work, we describe our recent efforts aimed at determining the mechanism of signal change for a diffraction-based sensor (DBS) system. The DBS detects analyte-binding events by monitoring the change in diffraction efficiency that takes place when analyte molecules adsorbs to target molecules that have been patterned onto a surface. The exact parameters that affect the intensity of the diffraction intensity are not immediately clear. In this work, it is hypothesized that the intensity of the diffraction signal depends both on the thickness of the diffraction grating and the refractive index of the analyte molecule. This hypothesis has been tested by preparing diffraction grating targets of well-defined thickness from polyelectrolyte multilayers. The index of refraction of the layers was also adjusted by incorporating charged Au nanoparticles into the diffraction grating structure. Results of these experiments are discussed in terms of simple models based on volume-phase holography.

Citation Download Citation

J. Valiani, J. Valiani, Matthew F. Paige, Matthew F. Paige, } "Investigation of the operating mechanism of a diffraction-based sensor", Proc. SPIE 6447, Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV, 64470M (12 February 2007); doi: 10.1117/12.698414; https://doi.org/10.1117/12.698414

ACCESS THE FULL ARTICLE

PERSONAL SIGN IN
Full access may be available with your subscription

Email or Username Forgot your username?

Password Forgot your password?

Show

Remember Email/Username on this computer

Remember Password

No SPIE account? Create an account
or Institutional Sign In via Shibboleth

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $14.40

Non-members: $18.00 ADD TO CART

Show All Keywords

Keywords/Phrases

Search In:

Publication Years