Colloid-gold nanoparticle enhanced detection sensitivity of paired surface plasma waves biosensor

Hsieh-Ting Wu; Yi-Ling Chen; Yen-Chen Huang; Wei-Chih Liu; Ming-Yaw Ng; Yin-Fong Chang; Bao-Yu Hsieh; Chien Chou

doi:10.1117/12.700009

12 February 2007 Colloid-gold nanoparticle enhanced detection sensitivity of paired surface plasma waves biosensor

Hsieh-Ting Wu, Yi-Ling Chen, Yen-Chen Huang, Wei-Chih Liu, Ming-Yaw Ng, Yin-Fong Chang, Bao-Yu Hsieh, Chien Chou

Proceedings Volume 6447, Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV; 64470S (2007) https://doi.org/10.1117/12.700009
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Previous work with amplitude-sensitive paired surface plasma waves biosensor (PSPWB) demonstrated that the detection sensitivity of PSPWB is able to experimentally detect 0.001% sucrose-water solution and biomolecular interaction of 10pg/ml mouse IgG interacting with immobilized anti-mouse IgG successfully. Further development of the detection sensitivity of PSPWB has been conducted by using 20nm in diameter colloid gold nanoparticles conjugated with target molecules that can result in a higher mass coverage and a larger resonant angle change of plasmon resonance, thereby its detection sensitivity is further enhanced significantly. Bare gold nanoparticles, which is randomly suspended in solution, is adopted to differentiate biospecific binding induced further signal enhancement. Experimentally, the sensitivity at 330fg/ml of Au-nanoparticle conjugated protein A (PA-Au) interacting with mouse IgG which is immobilized on a CM5 sensor chip was detected successfully. By this arrangement, 6-fold signal amplification is demonstrated compared with the same concentration of PA without conjugated gold nanoparticles.

Citation Download Citation

Hsieh-Ting Wu, Yi-Ling Chen, Yen-Chen Huang, Wei-Chih Liu, Ming-Yaw Ng, Yin-Fong Chang, Bao-Yu Hsieh, and Chien Chou "Colloid-gold nanoparticle enhanced detection sensitivity of paired surface plasma waves biosensor", Proc. SPIE 6447, Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV, 64470S (12 February 2007); https://doi.org/10.1117/12.700009

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available